Magnetic 3D Cell Culture Technology Levitation / Bioprinting / Bio Assay Greiner Bio-One

The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetisation of cells with NanoShuttle-PL. The magnetised cells can be aggregated by magnetic forces, either by levitation or bioprinting, to form microphysiologically relevant 3D models in vitro. The magnetic system overcomes the limitations of other platforms by enabling the manipulation of cells for the rapid formation of spheroids, reproducible and scalable in size for high-throughput, without restrictions of cell type.

• Mimicking native tissue environment
• Rapid 3D model formation within hours
• No specialized equipment, media, or artificial substrate
• Easy to handle / no sample loss
• Allows co-culture

Levitation 6 & 24 well

By levitating cells from the bottom of a cell-repellent plate, magnetic forces work as an invisible scaffold to gently and rapidly aggregate cells, forming larger spheroids at the air-liquid interface, induce cell-cell interactions and initiate ECM synthesis.

Bioprinting 96, 384 & 1536 well

With bioprinting magnetised cells are printed into spheroids by placing a cell-repellent plate atop a drive of magnets, where a single magnet below each well utilises mild magnetic forces to induce aggregation and print one spheroid at the bottom of each well within 15 minutes to a few hours.

Bio Assay 96 & 384 well

In addition bioprinting with a spheroidal shape, magnetic printing of cells can also be patterned into a ring formation. For up to 72 hours immediately following bioprinting, the patterned structures will shrink/close as a function of cell migration, viability, cell-cell interaction, and/or proliferation.