Efficient separation of lymphocytes and mononuclear cells from peripheral blood and bone marrow
- Enrichment directly from whole blood
- Simplified filling through porous barrier
- Rapid separation in 15 minutes at room temperature
- No additional laboratory equipment required
- Removal of erythrocytes and granulocytes
- No recontamination with erythrocytes
- No blocking of marker molecules
- Pre-filled option with Leucosep™ separation medium
- Available unfilled for usage of different separation media
Leucosep™ was developed for optimal separation of lymphocytes and peripheral mononuclear cells (so-called PBMCs) from human whole blood and bone marrow. The key feature of Leucosep™ is the porous barrier incorporated into the centrifuge tube made of highly translucent polypropylene. This barrier consists of highgrade polyethylene. It shows a precisely controlled pore size and does away with the time-consuming and laborious overlaying of the sample material. Anticoagulated blood or bone marrow can simply be poured directly from the blood sampling tube into the Leucosep™ tube. The porous barrier prevents mixture of the sample material with the separation medium. During centrifugation, lymphocytes and PBMCs are separated from unwanted erythrocytes and granulocytes on the basis of their density, and enriched in an interphase above the separation medium. When separation is complete, the barrier prevents recontamination of the enriched cell fraction during harvest. Leucosep™ may be used in combination with all common separation media for PBMC separation. For maximum convenience
Leucosep™ tubes are available as pre-filled tubes. The contained Leucosep™ separation medium has a density of 1.077 g/ml and yields excellent separation results.
Typical separation results with Leucosep™ separation medium:
Vitality
Viable cells [%] 95 ± 5
Cell yield
Lymphocytes [% of original number] 60 ± 20
Composition of enriched cell fraction
Mononuclear cells [%] 95 ± 5
Granulocytes [%] 5 ± 5
Erythrocytes [%] < 1
Composition of lymphocyte fraction
T cells [%] 83 ± 3
B cells [%] 6 ± 3
NK cells [%] 11 ± 2
as = aseptically produced
