Consumables / Accessories for 3D Cell Culture Kits Greiner Bio-One

Magnetic 3D Cell Culture

The core technology of Greiner Bio-One’s Magnetic 3D Cell Culture is the magnetisation of cells with NanoShuttle™-PL. The cells can be aggregated with magnetic forces, either by levitation or printing, to form structurally and biologically representative 3D models in vitro. NanoShuttle™-PL consists of gold, iron oxide, and Poly-L-Lysine. These nanoparticles (Ø < 50 nm) magnetise cells by electrostatically attaching
to cell membranes during an overnight static incubation. Magnetised cells will appear peppered with dark nanoparticles after incubation. NanoShuttle™-PL is biocompatible, having no effect on metabolism, proliferation and inflammatory stress. Additionally, it does not interfere with experimental techniques,
such as fluorescence or Western blotting. With magnetised spheroids, solution addition and removal is made easy by using magnetic force to hold them in a stationary position during aspiration, thereby limiting spheroid loss. Spheroids can also be picked up and transferred between vessels using magnetic tools such as the MagPen™ (7.657 850).


Advantages of magnetic cell culture:

• 3D in a 2D workflow
• Fast 3D tissue assembly
• Easy to handle / no sample loss
• Scalable – single well to 384 well
• Ease of co-culturing cells
• High-throughput Screening (HTS) with flat bottom for optical imaging
• Ready for automation
• Positioning spheroid centrally within the well